Info-enriched characterization of mobile populations. Morphological information from visuals can include to your richness of flow cytometry data. For instance, the determine shows an normally regular apoptosis assay employing Annexin V and PI, adding cell imaging to characterize cells in Every single population to reveal morphologically distinctive functions. These insights could not happen to be attained from multiplex staining alone.
No matter whether you’re a rookie or a professional, take a look at our sources to progress your exploration and abilities in flow cytometry.
Merge acoustic focusing technological innovation with significant-parameter Evaluation for adaptable spectral unmixing and traditional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating having a significant-speed brightfield digicam for simultaneous superior throughput flow cytometry and superior resolution brightfield imaging.
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Furthermore, it truly is illuminating to discover the amount of doublets slip as a result of regular singlet gates, and fantastic to confirm the position of unanticipated double-positive situations in my Assessment."
Optimizing gating technique. Even strong manual singlet gating is error-inclined and remains a subjective final decision stage in almost all flow cytometry assays. Imaging may be used to substantiate and modify gates to incorporate only single cells of desire.
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Early signs of this contamination experienced initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological features to find out appealing subpopulations that might not be apparent from flow cytometry data alone.
Most likely most importantly, these occasions include cells of Obviously different phenotypes which could lead on to incorrect conclusions relating to double good gatherings (especially in scarce populations).
Sound waves empower precise cell alignment, resulting in exceptional knowledge high-quality and ~10X more rapidly sample flow rates.
Software Be aware: Automated impression Assessment minimizes consumer-to-consumer variability in flow cytometry gating procedures
Constructed-in dimensionality reduction applications improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated populace resolution in lysed whole human blood. Consumer can look at situations from Every cluster for visual confirmation of mobile phenotypes.
No wash/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Professional UX/UI enables consumers to educate AI models for expanded applications. (A) The “Practice Model” aspect delivers annotation applications and true-time model schooling accuracy.
Gating on these prolonged parameters enables you to swiftly and accurately establish populations of curiosity and confirm gating system with little if any handbook overview. The Attune program delivers use of point out-of-the-art machine learning Investigation instruments inside an expertly created user interface, which makes it extremely simple to use.
Because the cell line experienced Beforehand been analyzed over the Attune CytPix Flow Cytometer, the scientists went again to the photographs and had been capable of document the microbial infection at least five times earlier.
The Attune CytPix Flow Cytometer makes it possible for end users to visualize samples throughout acquisition, confirm the precision of gating procedures, and assistance benefits with dependable documentation for publication reasons.
Here, we present that by gating procedures using picture-based mostly quantitative parameters can distinguish interacting cells from coincident activities to additional accurately examine interacting cells.